Search results for " Cyclic [administration & dosage]"

showing 10 items of 34 documents

Influence of dibutyryl cyclic AMP on thymidine uptake by herpes simplex virus infected cells and the intracellular level of cyclic AMP.

1977

Abstract Dibutyryl cyclic AMP inhibits the increase of dThd and BrdUrd transport normally observed after infection with Herpesvirus hominis, type I and II. Incorporation is also reduced. Inhibition of uptake is non-competitive as analysed by the Lineweaver-Burk plot. Addition of this drug to infected cells also reduces the activity of the thymidine kinase (EC 2.7.1.75). Transport of dUrd, dCyd and dAdo is not reduced. 4–8 h after infection with thymidine kinase (+) herpes strains the level of cAMP increases. On infection with a thymidine kinase (−) virus, only a small elevation of cAMP can be shown. It was also found that early addition of actinomycin D or of cycloheximide prevents the incr…

DNA ReplicationUltraviolet RaysDeoxyribonucleosidesBiologyCycloheximidemedicine.disease_causeVirus ReplicationBiochemistry Genetics and Molecular Biology (miscellaneous)Thymidine KinaseVirusCell Linechemistry.chemical_compoundSpecies SpecificitymedicineCyclic AMPSimplexvirusThymine NucleotidesCycloheximideDadoBiological TransportDibutyryl Cyclic AMPMolecular biologyKineticsHerpes simplex viruschemistryBromodeoxyuridineBucladesineThymidine kinaseDNA ViralDactinomycinThymidineIntracellularThymidineBiochimica et biophysica acta
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Fixed point theory for cyclic weak ϕ-contraction in fuzzy metric spaces

2012

In this paper, we introduce cyclic weak $\phi-$contractions in fuzzy metric spaces and utilize the same to prove some results on existence and uniqueness of fixed point in fuzzy metric spaces. Some related results are also proved besides furnishing illustrative examples.

Discrete mathematicsnon-Archimedean fuzzy metric spacFuzzy metric spaceInjective metric spacelcsh:QA299.6-433T-normEquivalence of metricslcsh:Analysiscyclic weak $phi-$contractionIntrinsic metricConvex metric spaceMetric spaceSettore MAT/05 - Analisi Matematicacyclic representationMetric mapFuzzy metric space cyclic representation cyclic weak ϕ-contraction non-Archimedean fuzzy metric spaceUltrametric spaceMathematics
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Cyclic behavior of composite truss beam-to-RC column joints in MRFS

2016

In the present paper, the cyclic behavior of beam-to-column joints made up of Hybrid Steel Trussed Concrete Beams (HSTCBs) connected to Reinforced Concrete (RC) piers is investigated. HSTCBs are a typology of composite beam generally constituted by a steel truss with end zone added rebars embedded within a block of concrete cast in place. HSTCBs represent a structural solution for light industrialization in the civil constructions; the main advantages in their use are the higher construction speed with the minimum site labor, the possibility of covering wide spans with low depths and a final economical convenience. For these reasons, they are also increasingly adopted within seismic framed …

EngineeringCyclic testComposite number0211 other engineering and technologiesTruss020101 civil engineering02 engineering and technologyComposite beams0201 civil engineeringColumn (typography)021105 building & constructionBeam-to-column joint; Composite truss beam; Cyclic tests; Seismic performance; Materials Science (all); Mechanics of Materials; Mechanical EngineeringGeneral Materials ScienceMechanics of MaterialDuctilityJoint (geology)Beam-to-column jointCyclic testsbusiness.industryComposite truss beamMechanical EngineeringStructural engineeringStrength of materialsSettore ICAR/09 - Tecnica Delle CostruzioniMechanics of MaterialsSeismic performanceMaterials Science (all)businessBeam (structure)
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Opposite effects of beta-adrenoceptor stimulation and 8-bromo-cyclic AMP on potassium efflux in mammalian heart muscle.

1980

beta-adrenoceptor stimulation by isoprenaline increases the potassium efflux in beating guinea-pig atria. This effect is not mimicked by 8-bromo-cyclic AMP, a cyclic AMP analogue which exerts a positive inotropic effect in this preparation.

Inotropemedicine.medical_specialtyAdrenergic receptorPotassiumGuinea Pigschemistry.chemical_elementAction PotentialsStimulationIn Vitro TechniquesCellular and Molecular Neuroscience8-Bromo Cyclic AMPInternal medicineIsoprenalineReceptors Adrenergic betamedicineCyclic AMPAnimalsMolecular BiologyPharmacologyChemistryMyocardiumIsoproterenolHeartCell BiologyMyocardial ContractionMammalian heartStimulation ChemicalReceptors AdrenergicEndocrinologycardiovascular systemPotassiumMolecular MedicineEffluxmedicine.drugExperientia
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Electrical and mechanical activity of mammalian heart muscle fibres treated with papaverine

1977

The action of papaverine on electrical and mechanical activity was investigated in ventricular and atrial heart muscle fibres from guinea-pigs and cats. 1. Papaverine (10−5 M–5×10−5M) had positive, negative or no inotropic effects in ventricular preparations; positive inotropic effects were not observed after pretreatment of the animals with reserpine. In atrial preparations, papaverine (2×10−5M) had a positive inotropic effect that was independent of endogenously stored catecholamines. 2. The effects of isoprenaline and dibutyryl cyclic AMP were potentiated by papaverine (10−5 M–2×10−5M). 3. The action potential duration was always prolonged by papaverine in ventricular as well as in atria…

Inotropemedicine.medical_specialtyReserpineGuinea PigsIn Vitro TechniquesMembrane PotentialsPapaverineIsoprenalineInternal medicinemedicineAnimalsDrug Interactionscardiovascular diseasesPharmacologyPapaverineCATSChemistryIsoproterenolHeartGeneral MedicinePapillary MusclesReserpineDibutyryl Cyclic AMPMyocardial ContractionMammalian heartEndocrinologyBucladesineCatscardiovascular systemAction potential durationmedicine.drugNaunyn-Schmiedeberg's Archives of Pharmacology
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Modulation of NMDA receptor function by cyclic AMP in cerebellar neurones in culture

2004

The signal transduction pathways involved in NMDA receptor modulation by other receptors remain unclear. cAMP could be involved in this modulation. The aim of this work was to analyse the contribution of cAMP to NMDA receptor modulation in cerebellar neurones in culture. Forskolin increases cAMP and results in increased intracellular calcium and cGMP that are prevented by blocking NMDA receptors. Similar effects were induced by two cAMP analogues, indicating that cAMP leads to NMDA receptor activation. It has been reported that phosphorylation of Ser897 of the NR1 subunit of NMDA receptors by cAMP-dependent protein kinase (PKA) activates the receptors. Forskolin increases Ser897 phosphoryla…

Intracellular Fluidmedicine.medical_specialty8-Bromo Cyclic Adenosine MonophosphateBiologyReceptors N-Methyl-D-AspartateBiochemistryCellular and Molecular Neurosciencechemistry.chemical_compoundCerebellumInternal medicineCyclic AMPmedicineAnimalsCyclic adenosine monophosphateNerve Growth FactorsEnzyme InhibitorsPhosphorylationRats WistarProtein kinase AReceptorLong-term depressionCyclic GMPCells CulturedNeuronsNeurotransmitter AgentsForskolinColforsinNeuropeptidesCyclic AMP-Dependent Protein KinasesRatsPituitary adenylate cyclase-activating peptideEndocrinologynervous systemchemistryPituitary Adenylate Cyclase-Activating PolypeptideNMDA receptorCalciumSignal transductionExcitatory Amino Acid AntagonistsSignal TransductionJournal of Neurochemistry
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Apelin treatment increases complete Fatty Acid oxidation, mitochondrial oxidative capacity, and biogenesis in muscle of insulin-resistant mice.

2012

Both acute and chronic apelin treatment have been shown to improve insulin sensitivity in mice. However, the effects of apelin on fatty acid oxidation (FAO) during obesity-related insulin resistance have not yet been addressed. Thus, the aim of the current study was to determine the impact of chronic treatment on lipid use, especially in skeletal muscles. High-fat diet (HFD)-induced obese and insulin-resistant mice treated by an apelin injection (0.1 μmol/kg/day i.p.) during 4 weeks had decreased fat mass, glycemia, and plasma levels of triglycerides and were protected from hyperinsulinemia compared with HFD PBS-treated mice. Indirect calorimetry experiments showed that apelin-treated mice…

MESH: Oxidation-Reduction[ SDV.AEN ] Life Sciences [q-bio]/Food and NutritionEndocrinology Diabetes and MetabolismGlucose uptakeAMP-Activated Protein KinasesInbred C57BLMice0302 clinical medicineAMP-activated protein kinaseMESH : Lipid MetabolismHyperinsulinemiaMESH: AnimalsMESH: AMP-Activated Protein KinasesMESH : Muscle SkeletalMESH : Fatty AcidsBeta oxidationMESH: Lipid Metabolism0303 health sciencesMESH: Muscle SkeletalbiologyMESH : Diet High-FatFatty AcidsMESH: Energy MetabolismMESH : AMP-Activated Protein KinasesMESH: Mitochondria MuscleSkeletal3. Good healthApelinMitochondriaMESH: Fatty AcidsMESH : Cyclic AMP-Dependent Protein KinasesMESH: Insulin ResistanceAlimentation et NutritionApelinIntercellular Signaling Peptides and ProteinsMuscleMESH : Insulin ResistanceOxidation-Reductionmedicine.medical_specialtyMESH : Mitochondria Muscle030209 endocrinology & metabolismMESH : Mice Inbred C57BLMESH: Cyclic AMP-Dependent Protein KinasesDiet High-Fat03 medical and health sciencesInsulin resistanceAdipokinesMESH: Mice Inbred C57BLInternal medicineMESH : MiceInternal MedicinemedicineFood and NutritionAnimalsMuscle SkeletalMESH: Intercellular Signaling Peptides and ProteinsMESH: MiceMESH : Intercellular Signaling Peptides and Proteins030304 developmental biologyMESH : Oxidation-ReductionAMPKmedicine.diseaseLipid MetabolismCyclic AMP-Dependent Protein KinasesMitochondria MuscleDietMice Inbred C57BLMESH : Energy Metabolism[SDV.AEN] Life Sciences [q-bio]/Food and NutritionAMP-Activated Protein Kinases;Animals;Cyclic AMP-Dependent Protein Kinases;Diet;High-Fat;Energy Metabolism;Fatty Acids;Insulin Resistance;Intercellular Signaling Peptides and Proteins;Lipid Metabolism;Mice;Inbred C57BL;Mitochondria;Muscle;Skeletal;Oxidation-ReductionHigh-FatMESH: Diet High-FatMetabolismEndocrinologyMitochondrial biogenesisbiology.proteinMESH : AnimalsInsulin ResistanceEnergy Metabolism[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
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GERMLINE PROKINETICIN RECEPTOR 2 (PROKR2) VARIANTS ASSOCIATED WITH CENTRAL HYPOGONADISM CAUSE DIFFERENTAL MODULATION OF DISTINCT INTRACELLULAR PATHWA…

2013

INTRODUCTION: Defects of prokineticin pathway affect the neuroendocrine control of reproduction, but their role in the pathogenesis of central hypogonadism remains undefined, and the functional impact of the missense PROKR2 variants has been incompletely characterized. MATERIAL AND METHODS: In a series of 246 idiopathic central hypogonadism patients, we found three novel (p.V158I, p.V334M, and p.N15TfsX30) and six already known (p.L173R, p.T260M, p.R268C, p.V274D, p.V331M, and p.H20MfsX23) germline variants in the PROKR2 gene. We evaluated the effects of seven missense alterations on two different prokineticin receptor 2 (PROKR2)-dependent pathways: inositol phosphate-Ca(2+) (Gq coupling) a…

MaleKallmann syndromeEndocrinology Diabetes and MetabolismClinical BiochemistryInositol Phosphatemedicine.disease_causeBiochemistryHypogonadotropic hypogonadismGermlineReceptors G-Protein-CoupledCohort StudiesEndocrinologySettore MED/38 - Pediatria Generale E SpecialisticaAdolescent; Adult; Child; Cohort Studies; Cyclic AMP; Female; Genetic Association Studies; Humans; Hypogonadism; Inositol Phosphates; Male; Middle Aged; Mutation Missense; Receptors G-Protein-Coupled; Receptors Peptide; Signal Transduction; Young Adult; Germ-Line MutationReceptorsCyclic AMPmutations; Kallmann syndrome; septo-optic dysplasiaMissense mutationReceptorChildMutationMiddle AgedProkineticinPeptideFemaleHumanSignal TransductionAdultmedicine.medical_specialtyReceptors PeptideAdolescentAdolescent Adult Child Cohort Studies Cyclic AMP; metabolism Female Genetic Association Studies Germ-Line Mutation Humans Hypogonadism; epidemiology/genetics Inositol Phosphates; metabolism Male Middle Aged Missense Receptors; G-Protein-Coupled; genetics Receptors; Peptide; genetics Signal Transduction; genetics Young AdultInositol PhosphatesMutation MissenseGenetic Association StudieBiologyG-Protein-CoupledYoung AdultGermline mutationInternal medicinesepto-optic dysplasiamedicineHumansGenetic Association StudiesGerm-Line MutationHypogonadismBiochemistry (medical)Kallmann syndromeProkineticin receptor 2medicine.diseasePROKR2 hypogonadism prokineticinmutationsAdolescent; Adult; Child; Cohort Studies; Cyclic AMP; Female; Genetic Association Studies; Humans; Hypogonadism; Inositol Phosphates; Male; Middle Aged; Mutation; Missense; Receptors; G-Protein-Coupled; Peptide; Signal Transduction; Young Adult; Germ-Line MutationEndocrinologyMutationCohort StudieMissense
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Demonstration of action-potential-producing cells in the rat pineal gland in vitro and their regulation by norepinephrine and nitric oxide

1998

There is evidence that sympathetically innervated mammalian pineal glands contain cells that exhibit action potentials. It is unknown whether ex vivo pineal glands deprived of their nervous input are still capable of firing. In the present study, multiple-unit recordings from rat pineals revealed spontaneously active cell clusters with a mean firing frequency of 1.5 +/- 0.3 Hz which could be abolished by tedrodotoxin. Regularly firing clusters showed no inherent periodicity in the minute range, whereas rhythmical clusters with periodically repeated bursts had period lengths of 12.6 min (day) and 9.5 min (night). Superfusion of norepinephrine reduced the firing frequency of both cluster type…

MaleNitroprussidemedicine.medical_specialtyPhysiologyPeriod (gene)8-Bromo Cyclic Adenosine MonophosphateAction PotentialsBiologyNitric OxideNitroargininePineal GlandNitric oxideRats Sprague-DawleyRat Pineal GlandNorepinephrine (medication)NorepinephrineBehavioral Neurosciencechemistry.chemical_compoundInternal medicinemedicineAnimalsSympathomimeticsCyclic GMPPhenylephrineInhibitory effectEcology Evolution Behavior and SystematicsNeuronsPenicillamineSulfhydryl ReagentsIsoproterenolIn vitroRatsElectrophysiologyEndocrinologychemistryAnimal Science and ZoologyEx vivomedicine.drugJournal of Comparative Physiology A: Sensory, Neural, and Behavioral Physiology
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Elevation in type I interferons inhibits HCN1 and slows cortical neuronal oscillations

2014

Central nervous system (CNS) inflammation involves the generation of inducible cytokines such as interferons (IFNs) and alterations in brain activity, yet the interplay of both is not well understood. Here, we show that in vivo elevation of IFNs by viral brain infection reduced hyperpolarization-activated currents (Ih) in cortical pyramidal neurons. In rodent brain slices directly exposed to type I IFNs, the hyperpolarization-activated cyclic nucleotide (HCN)-gated channel subunit HCN1 was specifically affected. The effect required an intact type I receptor (IFNAR) signaling cascade. Consistent with Ih inhibition, IFNs hyperpolarized the resting membrane potential, shifted the resonance fre…

MalePatch-Clamp TechniquesPotassium Channelsmedicine.medical_treatmentNeocortexInbred C57BLchemistry.chemical_compoundMiceReceptorsHyperpolarization-Activated Cyclic Nucleotide-Gated ChannelsReceptors InterferonMembrane potentialCerebral CortexNeuronsBlottingElectroencephalographyImmunohistochemistryCytokinemedicine.anatomical_structureInterferon Type IInterferonCytokinesSignal transductionWesternmedicine.drugSignal TransductionCognitive NeuroscienceCentral nervous systemBlotting WesternElectrophysiological ProcessesBiologyReal-Time Polymerase Chain ReactionTransfectionCellular and Molecular NeuroscienceCyclic nucleotidemedicineAnimalsHumansComputer SimulationIon channelNeuroinflammationInterferon-betaElectrophysiological PhenomenaRatsMice Inbred C57BLHEK293 CellschemistryNerve NetNeuroscienceInterferon type I
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